PMID27240257 Quantitative assessment of fluorescent proteins.
 Cranfill PJ1,2, Sell BR1, Baird MA1, Allen JR1, Lavagnino Z2,3, de Gruiter HM4, Kremers GJ4, Davidson MW1, Ustione A2,3, Piston DW
 Model bleaching as $log(F) = \alpha log(P) + c$ or $k_{bleach} = b I^{\alpha}$ where F is the fluorescence intensity, P is the illumination power, and b and c are constants.
 Most fluorescent proteins have $\alpha$ > 1, which means superlinear photobleaching  more power, bleaches faster.

 Catalog the degree to which each protein tends to form aggregates by tagging to the ER and measuring ER morphology. Fairly thorough  10k cells each FP.

PMID18204458 Highspeed, lowphotodamage nonlinear imaging using passive pulse splitters
 Core idea: take a single pulse and spread it out to $N= 2^k$ pulses using reflections and delay lines.
 Assume two optical processes, signal $S \propto I^{\alpha}$ and photobleaching/damage $D \propto I^{\beta}$ , $\beta \gt \alpha \gt 1$
 Then an $N$ pulse splitter requires $N^{11/\alpha}$ greater average power but reduces the damage by $N^{1\beta/\alpha}.$
 At constant signal, the same $N$ pulse splitter requires $\sqrt{N}$ more power, consistent with two photon excitation (proportional to the square of the intensity: N pulses of $\sqrt{N}/N$ intensity, 1/N per pulse fluorescence, $\Sigma \rightarrow 1$ overall fluorescence.)
 This allows for shorter dwell times, higher power at the sample, lower damage, slower photobleaching, and better SNR for fluorescently labeled slices.

 Examine the list of references too, e.g. "Multiphoton multifocal microscopy exploiting a diffractive optical element" (2003)
