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ref: -0 tags: adaptive optics sensorless retina fluorescence imaging optimization zernicke polynomials date: 11-15-2019 02:51 gmt revision:0 [head]

PMID-26819812 Wavefront sensorless adaptive optics fluorescence biomicroscope for in vivo retinal imaging in mice

  • Idea: use backscattered and fluorescence light to optimize the confocal image through imperfect optics ... and the lens of the mouse eye.
    • Optimization was based on hill-climbing / line search of each Zernicke polynomial term for the deformable mirror. (The mirror had to be characterized beforehand, naturally).
    • No guidestar was needed!
  • Were able to resolve the dendritic processes of EGFP labeled Thy1 ganglion cells and Cx3 glia.

{1249}
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ref: -0 tags: retinal ganglion cells neural encoding Farrow date: 07-31-2013 16:21 gmt revision:0 [head]

PMID-21273316 Physiological clustering of visual channels in the mouse retina

  • Anatomy predicts that mammalian retinas should have in excess of 12 physiological channels, each encoding a specific aspect of the visual scene.
  • Although several channels have been correlated with morphological cell types, the number of morphological types generally exceeds the known physiological types.
  • Here, we attempted to sort the ganglion cells of the mouse retina purely on a physiological basis.
  • Result: The optimal partition was the 12-cluster solution of the Fuzzy Gustafson-Kessel algorithm.
    • This might be useful elsewhere ...
  • Farrow Lab is responsible for the 11,011 electrode array.