PMID-20212151 Fusion of biomimetic stealth probes into lipid bilayer cores
- Used e-beam evaporation of Cr/Au/Cr 10/10/10 or 10/5/10 onto a Si AFM tip.
- Approx 200nm diameter; 1800 lipid interaction at the circumference.
- Exposed the Au in the sandwich via FIB
- Functionalized the Au with butanethiol or dodecanthiol; former is mobile on the surface, latter is polycrystaline.
- Butanethiol showed higher adhesion to the synthetic membranes
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- Measured the penetration force & displacement through synthetic multi-layer lipid bilayers.
- These were made via a custom protocol with 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (SOPC) and cholesterol
PMID-21469728 '''Molecular Structure Influences the Stability of Membrane Penetrating Biointerfaces.
- Surprisingly, hydrophobicity is found to be a secondary factor with monolayer crystallinity the major determinate of interface strength
- Previous studies using ellipsometry and IR spectroscopy have shown that alkanethiol self-assembled monolayers display an abrupt transition from a fluid to a crystalline phase between hexanethiol and octanethiol.
- This suggests the weakening of the membrane stealth probe interface is due to the crystallinity of the molecular surface with fluid, disordered monolayers promoting a high strength interface regime and rigid, crystalline SAMs forming weak interfaces.
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