Accelerated FRET-PAINT Microscopy
- Well isn't that smart -- they use a FRET donor, which is free to associate and dissociate form a host DNA strand, and a more-permanently attached DNA acceptor, which blinks due to FRET, for superresolution imaging.
- As FRET acceptors aren't subject to bleaching (or, perhaps, much less subject to bleaching), this eliminates that problem...
- However, the light levels used ~1kW / cm^2, does damage the short DNA oligos, which interferes with reversible association.
-
- Interestingly, CF488 donor showed very little photobleaching; DNA damage was instead the limiting problem.
- Are dyes that bleach more slowly better at exporting their singlet oxygen (?) or aberrant excited states (?) to neighboring molecules?
|