PMID-30997987 Chemistry of Photosensitive Fluorophores for Single-Molecule Localization Microscopy
- Excellent review of all the photo-convertable, photo-switchable, and more complex (photo-oxidation or reddening) of both proteins and small molecule fluorophore.
- E.g. PA-GFP is one of the best -- good photoactivation quantum yield, good N ~ 300
- Other small molecules, like Alexa Fluor 647 have a photon yield > 6700, which can be increased with triplet quenchers and antioxidants.
- Describes the chemical mechanism of the various photo switching -- review is targeted at (bio)chemists interested in getting into imaging.
- Emphasize that critical figures of merit are photoactivation quantum yield and N, overall photon yield before photobleaching.
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- See also Colorado lecture
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